4D trajectory optimization of commercial flight for green civil aviation

For the current development of green civil aviation, this study aims to optimize the green four-dimensional (4D) trajectory of commercial flight by taking into account conventional cost and environmental cost. Some fundamental models, efficient processing methodologies, and conventional objectives are proposed to construct the framework of trajectory optimization. Based on the environmental cost including greenhouse gas cost and harmful gas cost, green objective functions are presented. The A* algorithm and the trapezoidal collocation method are employed to optimize the lateral path and vertical profile for 4D optimization trajectory generation. A case study for the A320 from Barcelona Airport to Frankfurt Airport yields the results that the optimal costs can be obtained under different objectives and the total cost can be more optimized by adjusting the weights of environmental cost and conventional cost. The study builds an aided tool for 4D trajectory optimization and demonstrates that environmental factors and conventional factors should be taken into comprehensive consideration when constructing the flight trajectory in the future, as well as it can underpin the green and sustainable development of the air transport industry

Novel polyethyleneimine-R8-heparin nanogel for high-efficiency gene delivery <i>in vitro</i> and <i>in vivo</i>

<p>Gene therapy is an efficient and promising approach to treat malignant tumors. However, protecting the nucleic acid from degradation <i>in vivo</i> and efficient delivering it into tumor cells remain challenges that require to be addressed before gene therapy could be applied in clinic. In this study, we prepared novel polyethyleneimine-RRRRRRRR(R8)-heparin (HPR) nanogel as an efficient gene delivery system, which consists of heparin and cell penetrating peptide R8 grafted low-molecule-weight polyethyleneimine (PEI). Due to the shielding effect of heparin, crosslinking PEI-R8 with heparin was designed to diminish the toxicity of the gene delivery system. Meanwhile, a partial of R8 peptide which located on the surface of HPR nanogel could significantly enhance the cellular uptake. The formed HPR/pDNA complex exhibited effective endolysosomal escape, resulting in a high-efficiency transfection. Furthermore, the HPR could deliver the plasmid which could transcribe human TNF-related apoptosis inducing ligand (phTRAIL), into HCT-116 cells and induce significant cell apoptosis. In addition, HPR/phTRAIL complex showed satisfactory antitumor activity in abdominal metastatic colon carcinoma model. Finally, the antitumor mechanism of HPR/phTRAIL was also explored by western blot and histological analysis. The above results suggested that the HPR nanogel could serve as a promising gene delivery system.</p